SD-Met-Trp

Product introduction

2, English name SD-Met-Trp

3, Product properties: this product is white powder, odorless or slightly smelly

4, solubility: distilled water, 8g/L concentration

Yeast is a common model organism and is often used for genetic modification. Yeast amino acid deficient medium is necessary for yeast transformation and yeast hybridization. Its main uses include yeast single/yeast two-hybrid experimental research and yeast genetic mutant screening. According to the type of defective amino acids, it is divided into four types of media, namely one deficient, two deficient, three deficient, four deficient media, the company can customize the relevant media according to your requirements, quality assurance.

Two-deficient medium: this kind of yeast SC selective medium -SD medium is used for screening of double plasmid yeast transformation.

Uses: expression of foreign genes in yeast, functional complementation of heterologous genes, yeast one-hybrid system and yeast two-hybrid system initial plasmid transformation.

Coimmunoprecipitation, yeast Y187 and AH109 mating experiments, and mating type diploid screening.

For example: SD/-Trp-Leu, SD/-Trp-His, SD/-Trp-Ade, SD/-Leu-Ade, SD/-His-Ade, SD/-Leu-His, SD/-Ura-Trp, SD/-Ura-Leu, SD/-Ura-His, SC-Trp-Leu, SC-Trp-His, SC-Trp-Ade, SC-Leu-Ade, SC-His-Ade, SC-Leu-His, SC-Ura-Trp, SC-Ura-Leu, SC-Ura-His

Precautions

1: The medium contains all the required ingredients (except glucose, because some researchers need to use other sugars such as galactose to induce GAL1 promoter expression), a fine mixture of various amino acids and yeast nitrogenous base/yeast nitrogen source dry powder, weighed at 8g/L, and then prepared into the corresponding culture solution after adding water. No need to purchase MinimalSDBase or other yeast nitrogen source ingredients. After autoclaving, sterile glucose is added until the final concentration is 2% (or raffinose) or the specific inducer required by the experiment (such as the dose or concentration of galactose for the induced expression of GAL1 promoter is 2%), which is suitable for the expression of foreign genes in yeast. Selective defect cultures such as yeastone-Hybrid and yeasttwo-Hybrid systems screen for yeast phenotypes containing specific marker genes.

2: When preparing yeast defect medium, glucose can be added first and then high-pressure, which will not have a serious impact on the growth of yeast. However, if the addition of glucose and then high-pressure disinfection, the medium will change color, ranging from light yellow to dark brown, and still have a certain effect on the growth of cells (but under normal circumstances will not affect the experiment). Suggestion: Since amino acids and glucose can react chemically under high temperature and high pressure, the technical department does not recommend adding glucose to the post-high pressure, but recommends using the method indicated above 1 to prepare.

3 Suggestion: In principle, raffinose and galactose can not be high temperature and high pressure, because their chemical structure will change under high temperature and high pressure, which will inhibit the induction effect, which reminds researchers to pay attention to, especially when doing expression regulation mediated functional complementarity experiments!

4: When preparing a plate, that is, a soft Agar semi-solid medium (Agar powder 2%, i.e., 2 grams of Agar powder per 100ml) for conversion screening, the pH needs to be adjusted to about 6.0 before high pressure (for convenience, the pH range can vary between 5.6 and 6.5), while the liquid medium does not need to be adjusted.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.