ExonucleaseI,E.coli

Product Introduction

This enzyme has exonuclease activity that degrades single-stranded DNA from the 3 '-5' direction, progressively releasing DNA 5' monophosphate and leaving intact 5' terminal dinucleotides. The enzyme was derived from E.coli Exoi gene plasmid and purified by recombinant expression. This enzyme is mostly used to degrade digestion primers after PCR amplification. This enzyme is not active on double-stranded DNA and 3 'OH terminal DNA strands that are blocked by phosphoryl or acetyl groups.

Application

1. Remove primers from PCR mixture

2.PCR products were sequenced prior to PCR mutagenesis using large primers in a single tube

3. Remove the single strand of DNA containing the 3' hydroxyl terminus from the nucleic acid mixture

4. Detect the presence of a single strand of DNA containing the 3' hydroxyl terminus

Precautions

(1) 1×ExoIBuffer: 67mMGlycine-KOHpH9.5, 6.7mMMgCl2, 10mm2-mercaptoethanol. This enzyme is also active in conventional Pcrbuffers.

(2) The optimum reaction temperature of this enzyme is 37℃, and it can be inactivated at 80℃for 20min.

(3) The enzyme cannot cut double-stranded DNA, so single-stranded DNA containing secondary structures needs to be denatured before it can be fully digested.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.