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My Cart> Molecular Biology > PCR/qPCR > Nucleic Acid Amplification Enzyme & Premix > Bst4.0DNA/RNA Polymerase(8U/ul)(containing glycerin)
Storage：Store at -20℃，3 years.
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| Name | Bst4.0DNA/RNA Polymerase(8U/ul)(containing glycerin) |
| Storage | Store at -20℃，3 years. |
| Unit | Bottle |
| Specification | 1600U |
Product Introduction
Bst4.0DNA/RNAPolymerase is a mixture of Bst3.0DNA polymerase and the extremely heat-resistant ThermoStableVRTase reverse transcriptase (65℃tolerance), which is suitable for the LAMP reaction of RNA. Compared with Bst3.0 DNA/RNA polymerase, its reverse transcriptional activity is nearly 100 times higher and can detect low sensitivity RNA molecules. As the recommended enzyme in LAMP experiments using RNA as template, this enzyme has a higher amplification capacity than Bst3.0DNA/RNA polymerase. In addition, this enzyme can also perform LAMP amplification of DNA templates.
Precautions
(1) The used concentration of Mg2+ ranges from 4 to 10mM, and there is no Mg2+ in IsothermoBuffer. Generally, better LAMP results can be obtained under the condition of 6-8mMMg2+.
(2) It has been reported that adding TteUvrd helicase can improve the effect of LAMP.
(3) The specificity of amplification was tested using untemplated DNA as a control.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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