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My Cart> Molecular Biology > PCR/qPCR > Nucleic Acid Amplification Enzyme & Premix > Bst3.0DNA/RNA Polymerase(8U/ul)(containing glycerin)
Storage：Store at -20℃，3 years.
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| Name | Bst3.0DNA/RNA Polymerase(8U/ul)(containing glycerin) |
| Storage | Store at -20℃，3 years. |
| Unit | Bottle |
| Specification | 1600U |
Product Introduction
Compared with Bst2.0DNA polymerase and large fragments of BstDNA polymerase, Bst3.0DNA polymerase has better isothermal amplification activity and stronger reverse transcription activity. Whether using DNA or RNA as a template, this enzyme has 5 '-3' DNA polymerase activity and strong chain replacement activity, but the 5 '-3' and 3 '-5' exonuclease activity of this enzyme is missing.
In LAMP experiment using RNA as template, single enzyme system reaction can be realized. This enzyme has good reverse transcriptional activity between 60-65℃, which can effectively solve the reverse transcription of RNA templates with second-order complex structures, while Bst2.0DNA polymerase and large fragments of BstDNA polymerase have no such activity.
Precautions
(1) The used concentration of Mg2+ ranges from 4 to 10mM, and there is no Mg2+ in IsothermoBuffer. Generally, better LAMP results can be obtained under the condition of 6-8mMMg2+.
(2) It has been reported that adding TteUvrd helicase can improve the effect of LAMP.
(3) The specificity of amplification was tested using untemplated DNA as a control.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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