10000*SolarRed Nucleic acid dye

SolarRed is a sensitive, stable and relatively safe fluorescent nucleic acid staining reagent. It can replace the highly toxic stain Ethidium bromide (EB) for the staining of dsDNA and ssDNA in agarose gels or polyacrylamide gels. SolarRed is much more sensitive than EB and does not require decolorization. Since SolarRed and EB have the same spectral characteristics, there is no need to change the imaging system when replacing EB with it.

SolarRed can be used for both prefabricated gels and post-electrophoresis dyeing. Generally, the sensitivity of dyeing after electrophoresis is higher, and the interference of dye on DNA migration can be eliminated. Post-electrophoretic staining with SolarRed is simple and does not require decolorization or special solutions. As long as the dye is diluted in 0.1M NaCl and the gel is placed in the dyeing solution, it can be observed after 30min. The dye is extremely stable at room temperature and can be used repeatedly. In contrast, prefabricated gels require less dye and are simpler and more economical because they do not require additional dyeing processes.

In addition to its unparalleled sensitivity and stability, toxicity tests in independent laboratories have also shown that SolarRed is not mutagenic and cytotoxic at concentrations of gel staining. The key to SolarRed's safety is its complete lack of permeability to cell membranes.

Dye features:

1, Non-toxic: SolarRed's unique oiliness and large molecular weight characteristics make it unable to penetrate the cell membrane into the cell, Ames's test results also show that the mutation of the dye is far less than EB.

2, High sensitivity: suitable for electrophoresis staining of various size fragments, the impact on nucleic acid migration is less than SYBR Green I.

3, High stability: suitable for the use of microwave or other heating methods to prepare agarose gel; Extremely stable in acid or alkali buffers at room temperature, strong light resistance.

4, High signal-to-noise ratio: sample fluorescence signal is strong, low background signal.

5, Simple operation: like EB, the dye does not degrade in the process of preforming glue and electrophoresis; After electrophoresis, the dyeing process only takes 30 minutes without decolorization or washing, and can be directly observed by UV gel transmisometer.

6, Wide range of application: can choose before electrophoresis dyeing (glue dyeing) or after electrophoresis dyeing (bubble dyeing); Suitable for agarose gel or polyacrylamide gel electrophoresis; Can be used for dsDNA, ssDNA or RNA staining.

It has the same spectral characteristics as EB and does not need to change the filter and viewing device: the standard EB filter or SYBR filter is suitable, and the ordinary ultraviolet gel transmisometer is used to observe EB. However, SolarRed cannot be fully excited by 488 nm argon ion lasers or visible light of similar wavelengths, so imaging systems using such excitation devices are not recommended. For this type of device, we recommend that you use solarGreen (Cat# G5570), which has a similar spectrum to SYBR Green I, with comparable sensitivity, but is more stable.

Introduction to SolarRed

1. Glue dyeing method (same as EB)(recommended method)

(1) Add SolarRed nucleic acid dye when making glue (for example: add 5μL SolarRed 10,000× storage solution per 50mL agarose solution, and so on).

(2) Electrophoresis was performed according to conventional methods.

Note:

(1) The amount of dye used in this method is relatively small. 500 μL of dye can make about 100 pieces of 50mL glue.

(2) Because SolarRed has good thermal stability, it can be added directly in a hot agarose solution without waiting for the solution to cool. Shake, oscillate, or flip to ensure that the dye is well mixed. Alternatively, the SolarRed reservoir can be added to the agarose powder and electrophoresis buffer, and then heated in the microwave or other common method to prepare the agarose gel. SolarRed is compatible with almost all commonly used electrophoresis buffer solutions.

(3) If you always see band dispersion or separation is not ideal, it is recommended to use bubble dyeing to confirm whether the problem is related to the dye. If the problem persists after dyeing, the problem is not related to the dye, please try: reduce the agarose concentration; Use longer gels; Extend the gel time to ensure clear edges; Improve the sampling technique or choose blister dyeing method.

(4) This method is not suitable for prefabricated polyacrylamide gel, for polyacrylamide gel please use the foam dyeing method.

2. Soak dyeing method

(1) Electrophoresis was performed according to conventional methods.

(2) SolarRed 10,000× storage solution was diluted about 3,300 times with H2O into 0.1M NaCl to make 3× dyeing solution. (For example, add 15μL SolarRed 10,000× reservoir and 5mL 1M NaCl to 45mL H2O).

(3) Place the gel carefully into a suitable container, such as a polypropylene container. Slowly add a sufficient amount of 3× dye solution to immerse the gel. The dyeing time varies slightly according to the gel thickness and agarose concentration. For gels containing 3.5-10% acrylamide, the dyeing time is usually between 30min and 1h, and increases with the increase of acrylamide content.

Note:

(1) When dyeing with bubble dyeing, the amount of dye is more. A single use of the dye solution can be reused for about 3 times.

(2) 3×SolarRed dyeing solution can be prepared in large quantities and stored away from light at room temperature until used up.

Special reminder:

(1) If you are using an ultraviolet imager, please select SolarRed; If you use a laser imager or want to observe in visible light, choose SolarGreen.

(2) In rare cases where the DNA sample after the plasmid has been cut by certain enzymes will have a tailing and reduced resolution, it is recommended to try both staining methods at the same time to determine which method is more appropriate.

Note: Product information may be upgraded. Please refer to the actual label information.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.