{{cart_num}}
My Cart> Antibodies > Secondary Antibodies > Purified Secondary Antibodies > Rabbit anti-Chicken IgGReactivity：Chicken
Application： WB,ELISA
Appearance：Liquid
Storage：Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
Host：Rabbit
Qty：
Size：
| Name | Rabbit Anti-Chicken IgY（Purified Antibody） |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-20000.;ELISA:1:2000-100000. |
| Background | In chickens，immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG，it is composed of two light and two heavy chains. Structurally，these two types of immunoglobulin differ primarily in the heavy chains，which in IgY have a molecular mass of about 65，100 atomic mass units (amu)，and are thus larger than in IgG. The light chains in IgY，with a molar mass of about 18，700 amu，are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167，000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally，IgY is partially comparable to Immunoglobulin E (IgE)，as well as to IgG. However，in contrast to IgG，IgY does not bind to Protein A，to Protein G，or to cellular Fc receptors. Furthermore，IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem，after they were able to show differences between the immunoglobulins found in chicken eggs，and immunoglobulin G. Other synonymous names are Chicken IgG，Egg Yolk IgG，and 7S-IgG. |
| Immunogen | Chicken IgY full length protein |
| Calculated MW | 180kDa |
| Purification Method | Affinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
| Appearance | Liquid |
| Concentration | 10mg/mL |
| Unit | Piece |
| Y-Application | ELISA WB |
| Y-Dilution Ratio | WB 1:1000-20000.;ELISA:1:2000-100000. |
| Specification | 1mg 10mg 100mg |
| Reactivity | Chicken |
| Application | WB ELISA |
Product introduction：
The purified IgG antibody with high purity (>95%) and high activity was prepared by ammonium sulfate salting out and DEAE-ion-exchange cellulose chromatography, or batch adsorption purification. All kinds of purified antibodies are provided as liquid, which can be prepared in different concentrations according to user's requirements. It is best to store at -20℃ before use, and different concentrations can be diluted according to the use method. Usually different purposes can be diluted with the following buffer solution, pay attention to complete defrosting, fully mixed. Before use, it is best to remove the cold polymerized globulin and denatured protein by low temperature high-speed centrifugation, and then quantitate again.
Gold labeling: Diluted with pH9.0 borate buffer and labeled with 1-2 mg/ml.
HRP labeling: diluted with 0.01mol/L pH9.0, CB or coated solution CB, 6-8 mg/ml labeling
Fluorescent labeling: diluted with 0.5mol/L pH9.5, CB(carbonate buffer), 10-20 mg/ml labeling.
Biotinylated antibody: diluted with 0.1mol/L NaHCO3 and labeled with 1-2 mg/ml. Other methods, such as immunodiffusion, can be diluted with 0.01mol/L pH7.2 PBS and coated with 10-20 μg/ml ELISA at 4℃ overnight or 37℃ for 2 hours.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
