Lactic acid（LA） content Assay Kit
Cat.No：BC5345 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Lactic acid（LA） content Assay Kit
Product Information
| Name | Lactic acid（LA） content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Lactic acid is an important intermediate product in the process of biological metabolism, which is closely related to glucose metabolism, lipid metabolism, protein metabolism and intracellular energy metabolism. Lactic acid content is an important index to evaluate glycogen metabolism and aerobic metabolism.
Measurement Principle：
Under the action of Lactate dehydrogenase, pyruvate is generated from Lactic acid, and NAD+ is reduced to produce NADH and H+. Under the action of 1-mPMS, WST-1 reacts with NADH to produce water-soluble formazan, which has the maximum absorption peak at 450nm, based on which the content of Lactic acid can be calculated.
Self Provided：
Spectrophotometer/Microplate reader, mortar/homogenizer/cell ultrasonic crusher, centrifuge, constant temperature foster box/water-bath, centrifuge, micro quartz cuvette/96 well flat-bottom plate, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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