NKH477
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Cat.No：IN1860 Solarbio
CAS：138605-00-2
Storage：Powder:2-8℃，2 years
Purity：≥98%
Appearance：White to off-white Solid
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> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > GPCR & G Protein > NKH477
Product Information
| CAS | 138605-00-2 |
| Name | NKH477 |
| Molecular Formula | C27H44ClNO8 |
| Molecular Weight | 546.09 |
| Solubility | Soluble in Water/DMSO(Need ultrasonic)(This product is not stable in solution, it is recommended that you use it now) |
| Purity | ≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years |
| SMILES | CC(=O)O[C@H]1[C@H]([C@@H]2[C@]([C@H](CCC2(C)C)O)([C@@]3([C@@]1(O[C@@](CC3=O)(C)C=C)C)O)C)OC(=O)CCN(C)C.Cl |
| InChIKey | VIRRLEDAYYYTOD-YHEOSNBFSA-N |
| InChI | InChI=1S/C27H43NO8.ClH/c1-10-24(5)15-18(31)27(33)25(6)17(30)11-13-23(3，4)21(25)20(35-19(32)12-14-28(8)9)22(34-16(2)29)26(27，7)36-24;/h10，17，20-22，30，33H，1，11-15H2，2-9H3;1H/t17-，20-，21-，22-，24-，25-，26+，27-;/m0./s1 |
| PubChem CID | 444028 |
| Target Point | Adenylate Cyclase |
| Passage | GPCR & G Protein |
| Background | NKH477 directly activates the catalytic unit of adenylate cyclase and increases intracellular cAMP. |
| Biological Activity | NKH477 可直接激活腺苷酸环化酶的催化单元并增加细胞内的 cAMP。NKH477 是一种毛喉素衍生物，通过对心脏舒张功能的有益影响来改善心力衰竭。NKH477 通在大鼠体内对细胞有抗增殖作用，从而抑制大鼠异位肺移植的急性排斥反应。[1] |
| In Vivo | NKH477 exerts an antiproliferative effect on lymphocytes in vivo with an altered cytokine profile in rat recipients of lung allografts. NKH477 significantly extended allograft survival time in a dose-dependent manner and reduced histopathological rejection. Treatment with NKH477 inhibited IFN-gamma and IL-10 expression. NKH477 suppressed expression of both cytokines in the spleen.[1] |
| Animal Experiment | Left lungs were transplanted orthotopically from Brown-Norway donors to Lewis recipients. Recipient rats were untreated or treated daily with different doses of NKH477. Grafts were excised on Day 3 or Day 5 to determine histopathological rejection and expressions of interleukin(IL)-2，IL-4，IL-10，and interferon(IFN)-gamma by enzyme-linked immunosorbent assay. The cytokine expression at Day 3 or Day 5 was also evaluated in recipient spleens by immunohistochemistry. Furthermore，mesenteric lymph node cells from recipients at Day 5 were cultured alone or stimulated with donor antigens for 72 hours to determine cell proliferation by means of thymidine incorporation.[1] |
| Data Literature Source | [1]. Nakashima S，et al. Antiproliferative effects of NKH477，a forskolin derivative，on cytokine profile in rat lung allografts. J Heart Lung Transplant. 2005 Apr;24(4):462-9. |
| Unit | Bottle |
| Specification | 5mg |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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