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My CartCAS：11070-73-8
Storage：Powder:-20℃，1 year;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Appearance：White to off-white Solid
Qty：
Size：
| CAS | 11070-73-8 |
| Name | Insulin from bovine pancreas |
| Molecular Formula | No data available |
| Solubility | Soluble in Water ≥10 mg/mL(Need ultrasonic and add HCl to help dissolve) |
| Enzyme Activity/Titer | ≥27U/mg |
| Source | 牛胰腺 |
| Appearance | White to off-white Solid |
| Storage | Powder:-20℃，1 year;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 234-291-2 |
| MDL | MFCD00131380 |
| SMILES | CC[C@H](C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC( =O)O)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C @H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H ](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H] (CSSC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[ C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@ @H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CSSC[C@@H ](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N2)C(C)C )CO)C)NC1=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC3=CN=CN3)NC(=O)[C @H](CCC(=O)N)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](C(C)C)NC(=O )[C@H](CC4=CC=CC=C4)N)CO)CC5=CN=CN5)CC(C)C)C(C)C)CCC(=O)O)C)CC(C) C)CC6=CC=C(C=C6)O)CC(C)C)C(C)C)C(=O)NCC(=O)N[C@@H](CCC(=O)O )C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](CC7=CC=CC=C7)C(= O)N[C@@H](CC8=CC=CC=C8)C(=O)N[C@@H](CC9=CC=C(C=C9)O)C(=O)N[ C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[ C@@H](C)C(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)CC1=CC=C(C=C1)O)CC(=O) N)CCC(=O)O)CC(C)C)CCC(=O)N)CC1=CC=C(C=C1)O)CC(C)C)CO)NC(=O)CN |
| InChIKey | CC[C@H](C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC( =O)O)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C @H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H ](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H] (CSSC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[ C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@ @H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CSSC[C@@H ](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N2)C(C)C )CO)C)NC1=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC3=CN=CN3)NC(=O)[C @H](CCC(=O)N)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](C(C)C)NC(=O )[C@H](CC4=CC=CC=C4)N)CO)CC5=CN=CN5)CC(C)C)C(C)C)CCC(=O)O)C)CC(C) C)CC6=CC=C(C=C6)O)CC(C)C)C(C)C)C(=O)NCC(=O)N[C@@H](CCC(=O)O )C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](CC7=CC=CC=C7)C(= O)N[C@@H](CC8=CC=CC=C8)C(=O)N[C@@H](CC9=CC=C(C=C9)O)C(=O)N[ C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[ C@@H](C)C(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)CC1=CC=C(C=C1)O)CC(=O) N)CCC(=O)O)CC(C)C)CCC(=O)N)CC1=CC=C(C=C1)O)CC(C)C)CO)NC(=O)CN |
| InChI | InChI=1S/C254H377N65O75S6/c1-29-131(24)205(312-192(334)103-256)250(389)317-204(130(22)23)246(385)287-158(74- 81-199(344)345)216(355)281-155(70-77-188(260)330)220(359)307-183-116-399-400-117-184-242(381)305- 177(110-321)238(377)293-161(87-122(6)7)223(362)294-167(94-139-52-60-145(324)61-53-139)226( 365)282-153(68-75-186(258)328)217(356)289-160(86-121(4)5)221(360)284-157(73-80-198(342)343) 219(358)301-173(100-189(261)331)233(372)297-169(96-141-56-64-147(326)65-57-141)229(368)308-182( 241(380)303-175(253(393)394)102-191(263)333)115-398-396-113-180(212(351)270-106-193(335)277-152(71- 78-196(338)339)215(354)280-150(50-41-83-268-254(264)265)210(349)269-107-194(336)278-165(92-137- 45-35-31-36-46-137)225(364)296-166(93-138-47-37-32-38-48-138)228(367)298-170(97-142-58- 66-148(327)67-59-142)236(375)318-206(135(28)323)251(390)319-84-42-51-185(319)244(383)285-151( 49-39-40-82-255)213(352)276-134(27)252(391)392)310-248(387)202(128(18)19)315-234(373)163(89-124(10) )11)291-227(366)168(95-140-54-62-146(325)63-55-140)295-222(361)159(85-120(2)3)288-207(346 )132(25)274-214(353)156(72-79-197(340)341)286-245(384)201(127(16)17)314-235(374)164(90-125(12) )13)292-231(370)172(99-144-105-267-119-273-144)300-237(376)176(109-320)279-195(337)108-271-211(350) )179(112-395-397-114-181(309-243(183)382)240(379)275-133(26)208(347)304-178(111-322)239(378)316-203 (129(20)21)249(388)311-184)306-224(363)162(88-123(8)9)290-230(369)171(98-143-104-266-118-272 -143)299-218(357)154(69-76-187(259)329)283-232(371)174(101-190(262)332)302-247(386)200(126(14)15 )313-209(348)149(257)91-136-43-33-30-34-44-136/h30-38，43-48，52-67，104-105，118-135，149-185，200-206，320-327H，29 ，39-42，49-51，68-103，106-117，255-257H2，1-28H3，(H2，258，328)(H2，259，329)(H2，260，330)(H2，261，331)(H2，262，332)(H2，263，333)(H，266，272)(H，267，273)(H，269，349)(H，270，351)(H，271，350)(H，274，353)(H，275，379)(H， 276，352)(H，277，335)(H，278，336)(H，279，337)(H，280，354)(H，281，355)(H，282，365)(H，283，371)(H，284，360)(H，285，383)(H， 286，384)(H，287，385)(H，288，346)(H，289，356)(H，290，369)(H，291，366)(H，292，370)(H，293，377)(H，294，362)(H，295，361)(H， 296，364)(H，297，372)(H，298，367)(H，299，357)(H，300，376)(H，301，358)(H，302，386)(H，303，380)(H，304，347)(H，305，381)(H， 306，363)(H，307，359)(H，308，368)(H，309，382)(H，310，387)(H，311，388)(H，312，334)(H，313，348)(H，314，374)(H，315，373)(H， 316，378)(H，317，389)(H，318，375)(H，338，339)(H，340，341)(H，342，343)(H，344，345)(H，391，392)(H，393，394)(H4，264，265，268)/t131- ，132-，133-，134-，135+，149-，150-，151-，152-，153-，154-，155-，156-，157-，158-，159-，160-，161 -，162-，163-，164-，165-，166-，167-，168-，169-，170-，171-，172-，173-，174-，175-，176-，177-， 178-，179-，180-，181-，182-，183-，184-，185-，200-，201-，202-，203-，204-，205-，206-/m0/s1 |
| PubChem CID | 16131099 |
| Target Point | Insulin Receptor |
| Passage | Endocrinology & Hormones |
| Background | Insulin is a protein-like hormone that is secreted by pancreatic beta cells and has the activity of lowering blood sugar. This product is derived from bovine pancreas. |
| Unit | Bottle |
| Specification | 25mg 100mg |
Examples of using this product（for reference only）
In Vitro:
Cell （Adipogenic differentiation；1.9 μM Insulin,2d；10 μg/mL Insulin,14d/change the medium every two days）:
The resuscitated cells were seeded in 12-well plates for culture, and the medium was changed every other day until the cell confluency reached 70?80%. For adipogenic differentiation, the differentiation medium was replaced. The differentiation medium contained 10% FBS, 1 mM 3- isobutyl-1-methylxanthine, 1 μM dexamethasone, and 1.9 μM insulin (insulin from bovine pancreas, Solarbio, China) in DMEM. This process lasted for two days, and then, the medium was changed to adipocyte maintenance medium (DMEM supplemented with 10% FBS and 10 μg/mL insulin) and cultured for 14 days (change the medium every two days). The differentiated cells were harvested and fixed for 30 min with 4%
paraformaldehyde. The fixed cells were stained with oil red O for 60 min. The stained cells were washed three times with isopropanol for further examination under an inverted fluorescence microscope. Five images of the cells after cryopreservation and differentiation of fresh cells were selected, and the differentiated and undifferentiated cells were counted and the differentiation efficiency was calculated.
Reference：
Xia Y, Huang LX, Chen H, Li J, Chen KK, Hu H, Wang FB, Ding Z, Guo SS. Acoustic Droplet Vitrification Method for High-Efficiency Preservation of Rare Cells. ACS Appl Mater Interfaces. 2021 Mar 24;13(11):12950-12959. doi: 10.1021/acsami.1c01452. Epub 2021 Mar 11. PMID: 33703892.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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