Chloroplast Protein Extraction Kit (For proteome test, mass spectrometry)-Enzymatic method

Chloroplasts are unique organelles for energy conversion in plant cells, and photosynthesis is carried out in chloroplasts. Because of this important function, chloroplasts have been the important research objects in cell biology, genetics and molecular biology. Chloroplast extraction kit The chloroplast total protein can be quickly extracted from various plant samples by a simple and rapid method. This kit is suitable for extracting chloroplast protein from fresh plant samples. The recovery rate of chloroplast protein is low due to the destruction of most chloroplast cells in the frozen storage process. This kit contains a unique formulation that effectively dissolves chloroplast membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The chloroplasts prepared by enzymatic extraction were obtained with higher recovery and higher purity, but it took a long time. The extraction process of the non-enzymatic extraction kit is simple and convenient, fast, can be completed within 1 hour, and there is little cross-contamination, but the recovery rate is lower than that of the enzymatic method. If you need a faster extraction kit, you can choose a non-enzymatic extraction kit, and if there is no requirement for the extraction speed, you can choose an enzymatic extraction kit. Select a kit as required. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.