Plant Nucleoprotein Extraction Kit (Enzyme method, pull-down application)

Plant nuclear protein extraction kit provides a complete set of reagents suitable for extracting nuclear protein from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient. The prepared nucleoprotein not only has high purity and natural activity, but also has little cross contamination. This kit contains a unique formula that effectively dissolves plant nuclear components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. This kit is composed of optimized reagent formulation, which is especially suitable for downstream protein interaction research experiments, including immunoprecipitation (IP), immunoco-precipitation (co-IP), Pull-down and other experiments. The protein extracted from this kit can also be used for WesternBlotting, protein electrophoresis, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. This kit is extracted by enzymatic method. The plant nuclear protein prepared by enzymatic method has high recovery rate, high purity and natural activity, but it takes a long time. The extraction process of the non-enzymatic extraction kit is simple and convenient, fast, can be completed within 1 hour, and there is little cross-contamination, but the recovery rate is lower than that of the enzymatic method. If you need a faster extraction kit, you can choose a non-enzymatic extraction kit, and if there is no requirement for the extraction speed, you can choose an enzymatic extraction kit. Select a kit as required. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: plants

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.