Endoplasmic Reticulum Protein Extraction Kit (Low speed centrifugation)

The endoplasmic reticulum is present in all kinds of eukaryotic cells except mature red blood cells of mammals. The endoplasmic reticulum (ER) is a system of interconnected lamellar gaps or small tubules composed of biofilms. The gap space between the membranes is called the pool, which is usually not directly connected with the extracellular Spaces and the cytoplasmic matrix. On the one hand, this membranous duct system constitutes the transport pathway of intracellular substances, on the other hand, it provides a broad reaction area for various enzyme reactions in the cell. The functions of the endoplasmic reticulum are related to the synthesis of proteins, the synthesis of sugars and lipids, detoxification and assimilation, and also have the function of transporting proteins. The traditional extraction methods of endoplasmic reticulum protein require high centrifugal force or density gradient centrifugation, which requires high requirements for laboratory centrifuge equipment and high requirements for experimental operation detail. If the laboratory does not have equipment that can reach a centrifugal force of more than 50,000 ×g, it cannot be extracted. Endoplasmic reticulum protein extraction kit (low-speed centrifugal method) only needs to reach the centrifugal force within 20000×g to extract endoplasmic reticulum protein, and the general conventional desktop centrifuge can meet the requirements of use. The ER protein extraction kit can be used to extract ER protein from various animal cells and solid soft and hard tissue samples. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: animal cells/tissues

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.