Primary Cell Plasmid DNA Transfection Kit

Product description:

The high efficient nucleic acid cotransfection kit is specially used to co-transfect plasmids DNA, siRNA or mimics into the same cell. The product has excellent transfection performance and can efficiently co-transfect plasmid DNA and siRNA into most adherent cells and primary cells. In adherent cells, the transfection efficiency of plasmid DNA can reach more than 90%, and the co-transfection efficiency of siRNA in plasmid transfection positive cells can be as high as 95%. The product has powerful functions, which can efficiently transfect not only plasmids DNA and siRNA, but also mRNA, mimics, antisense oligodeoxynucleotides and various small molecule DNA. Different from the common liposome transfection reagents in the market, the product is prepared with biodegradable materials and has low toxicity to cells, and there is almost no obvious cell death 24 hours after transfection. It is also very convenient to use. First, the transfection reagent was mixed with plasmids DNA and siRNA, and then the transfection complex was directly added to the cultured cells. The serum does not affect the transfection effect, and there is no need to deliberately add serum or replace the culture medium. The operation is very simple.

Product characteristics:

The excellent co-transfection performance of plasmid DNA and siRNA, it can transfect a variety of adherent cells and primary cells efficiently. The transfection efficiency of plasmid DNA in adherent cells is more than 90%, and the co-transfection efficiency of siRNA in plasmid positive cells is more than 95%;

The co-transfection is powerful, it can not only co-transfect plasmid DNA and siRNA in the same cell, but also co-transfect mRNA, mimics, antisense nucleic acid in the same cell;

Extremely low cytotoxicity: the use of biodegradable biomaterials has low cytotoxicity and the death rate of transfected cells is less than 10%, which greatly reduces the effect of cytotoxicity on the experimental results;

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.