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Cysteinyl Sulfoxide Lyase （CSL）Activity Assay Kit

BC5395Manual

Cat.No：BC5395 Solarbio

Storage：Store at 2-8℃，6 months

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Product Information

Name	Cysteinyl Sulfoxide Lyase （CSL）Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

"Cysteinyl sulfoxide lyase, abbreviated as alliinase, also known as alliinase. Cysteyl sulfoxide lyase is present in almost all allium plants, such as garlic, onions, leeks, etc. Alliinase is present inside the vacuole, and its natural substrate, alliin, is present in the cytoplasm; Alliinase comes into contact with alliin and catalyzes the production of allicin and cis- and trans-ahorene and produces by-products such as pyruvate and ammonia, which is also the main source of the pungent smell of plants such as garlic.

Measurement Principle：

Cysteamide sulfoxide lyase catalyzes the formation of pyruvate from S-methyl-L-cysteine sulfoxide. Pyruvate can react with 2,4-dinitrophenylhydrazine to form 2,4-dinitrophenylhydrazone, which is brownish-red under alkaline conditions. The change in absorbance at 505 nm is measured to calculate the CSL enzyme activity.

Self Provided：

Visible spectrophotometer/microplate reader, water bath/incubator, tabletop centrifuge, adjustable pipette, microglass cuvette/96-well plate, mortar/homogenizer, distilled water, and ice.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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