Pyrroline-5-Carboxylate Reductase（P5CR）Activity Assay Kit
Cat.No：BC5380 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Other Biochemical Series > Other Series > Pyrroline-5-Carboxylate Reductase（P5CR）Activity Assay Kit
Product Information
| Name | Pyrroline-5-Carboxylate Reductase（P5CR）Activity Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 50T/24S |
Determination of Significance：
Pyrroline-5-carboxylate reductase (P5CR) is an important housekeeping protein widely found in prokaryotes and eukaryotes. In the presence of NAD(P)H, pyrroline-5-carboxylic acid (P5C) is converted to proline by pyrroline-5-carboxylate reductase, and P5CR has also been found to be involved in the metabolism of thioproline in E. coli.
Measurement Principle：
Thioproline is dehydrogenated by pyrroline-5-carboxylate reductase and is accompanied by the conversion of NAD to NADH. In the presence of 1-mPMS, WST-1 reacts with NADH to produce water-soluble formazan with a characteristic absorption peak at 450 nm.
Self Provided：
Spectrophotometer, water bath/constant incubator, adjustable pipette, analytical balance, mortar/homogenizer/cell sonicator , 1mL glass cuvette, distilled water and ice.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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