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Glutamine (Gln) Content Assay Kit

BC5300Manual

Cat.No：BC5300 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Reagents Required But Not Provided	Please check the manual first, there are some reagents you should prepare by yourself.
Name	Glutamine (Gln) Content Assay Kit
Detection Method	Spectrophotometer
Storage	Store at -20℃，6 months
Unit	Kit
Specification	50T/24S

Determination of Significance：

Glutamine is the amide of glutamic acid and one of the important amino acids that make up proteins. At the same time, glutamine is also the main source of α-ketoglutaric acid in the tricarboxylic acid cycle. Glutamine exists in the organism in two states: free state and bound state. Free glutamine plays an important role in organism metabolism, and its metabolism accounts for more than 60% of free amino acids in cells and blood circulation.

Measurement Principle：

Free glutamine is converted to glutamic acid under the catalysis of glutaminase, Under the catalysis of glutamate dehydrogenase (GDH), glutamate and NAD are produced α- Ketoglutaric acid, NADH and NH4+, under the action of 1-mPMS, WST can react with NADH to produce water-soluble formazan, which has the maximum absorption peak at 450 nm, and the glutamine content can be calculated.

Self Provided：

Spectrophotometer, Desktop Centrifuge, Water Bath/Constant Temperature Incubator, Transferpettor, Mortar/Homogenizer/Cell Ultrasonic Crusher, 1 mL Glass Cuvette, Chloroform and Distilled Water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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