DNA Extraction Phenol Reagent
Cat.No：T0250 Solarbio
Storage：Store at 2-8℃，avoid light，1 year.
Appearance：Light Yellow Clarifies Liquid
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> Molecular Biology > Nucleic Acid Extraction/Purification > DNA/RNA Extraction > DNA Extraction Phenol Reagent
Product Information
| Name | DNA Extraction Phenol Reagent |
| Appearance | Light Yellow Clarifies Liquid |
| Storage | Store at 2-8℃，avoid light，1 year. |
| Unit | Bottle |
| Specification | 250ml |
Product Description: This reagent is light yellow transparent liquid, with pungent odor, the upper layer is Tris hydrochloric acid buffer, pH>7.8, the lower layer is phenol phase, and the antioxidant 8-hydroxyquinoline is added. This product is suitable for isolating DNA.
Usage: Tissue cracking solution, ensure that the pH is about 8.0 (0.1 volume of 1M Tris-HCl can be added, pH8.0, adjust it to about 8.0), and then add 0.5 volume of DNA phenol extraction reagent (stand when using, take the lower phenol phase for use. The upper layer is Tris-HCl solution, which is to prevent the phenol from contact with the air to slow the oxidation of the phenol), 0.5 volume of chloroform: isoamyl alcohol (24∶1), and ice bath for 15 minutes after shaking for 10s. After centrifugation, take water phase (upper layer) and add equal volume of isopropyl alcohol to precipitate DNA, rinse with 75% ethanol once, slightly dry and dissolve in appropriate amount of TE for use.
Note: DNA extraction phenol reagent has strong corrosive, should try to avoid skin contact or inhalation in the body. If it is found to be red or brown, it indicates that oxidation has occurred and cannot be continued.
Solubility: 10 mg/mL in water
Note: Product information may be upgraded. The actual label information prevails.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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