UIP
Cat.No：P2070 Solarbio
Storage：Store at -80℃，transport with dry ice.
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | UIP |
| Storage | Store at -80℃，transport with dry ice. |
| Enzyme Activity/Titer | 5U/ul |
| Unit | Bottle |
| Specification | 1000U(200μL) |
Product introduction
The SUMO protease, also known as Ulp, is a highly active cysteine protease that recognizes the tertiary structure of the SUMO protein rather than the amino acid sequence, thus efficiently and specifically cutting the SUMO protein from the recombinant fusion protein.SUMO protease can maintain high activity in a wide range of reaction environmental systems. SUMO protease carries a polymerized His label, which facilitates the removal of the protease by affinity chromatography after cutting the fusion protein.
Usage method
1. 10×SUMO Protease Buffer: 500mM Tris-HCl, 10mM DTT, pH 8.0.
2. The ratio of SUMO protease to the target protein requiring enzyme digestion: 1:100.
3. Enzyme cutting system:
Fusion protein 1000 μg
10×SUMO Protease Buffer 20μL
SUMO protease 2μL
ddH2O constant volume to 1000μL
4. Enzyme cutting conditions: It is recommended to cut overnight at 4℃, and users can explore according to the target protein of their research. The following enzyme cutting analysis pictures are for reference.
5. A small number of samples can be used for SDS-PAGE analysis after enzyme digestion. To remove SUMO protease in the system after enzyme digestion, His label can be used to purify the tree
Unit definition
In a 1× SUMO Protease Buffer (50 mM Tris-HCl, 1 mM DTT, pH 8.0) reaction at 30℃ for 1h, the amount of enzyme required to shear more than 85% of the 2 μg substrate was defined as a unit of activity.
Optimum pH
5.5-9.5
Optimum temperature
4-30℃
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no experimental images.
Related Products
Sorry, there is no more information.
Related Paper
Melatonin alleviates cadmium-induced nonalcoholic fatty liver disease in ducks by alleviating autophagic flow arrest via PPAR-α and reducing oxidative stress
Click to check >>Author：Chang Liu, Hong Lin, Limin Cao, Kaiqiang Wang, Jianxin Sui
IF：6.1000
Publish_to：TALANTA
PMID：37385191
Technical Q&A
Q&A
（Total 0
User Q&A）
- There is not question and answer now, come and send an inquiry please.
My question
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: [email protected]
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.