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Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
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Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
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GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
FAQ—Solarbio Microbial Media
FAQ Documentation—Microbial Media
| Q01: | Does microbial media come with an instruction manual? |
| A01: | Typically, microbial media do not come with a separate instruction manual. The components and usage instructions are instead listed on the label of the bottle. |
| Q02: | What is the product code for microbial media? |
| A02: | Most product codes for microbial media begin with "LA". |
| Q03: | Can microbial media be customized? |
| A03: | Due to the complex manufacturing process involved in producing microbial media, customization to meet specific customer requirements is currently not feasible. |
| Q04: | Preliminary pH adjustment of media |
| A04: | Since the pH of media can change during the heating and sterilization process, a preliminary pH adjustment should be performed after all components have fully dissolved. For instance, beef extract may lower the pH by approximately 0.2, while intestinal extract may significantly increase it. Therefore, operators should pay close attention to developing experience in this step to ensure accurate final pH levels and maintain media quality. Following pH adjustment, the media should be boiled for several minutes to facilitate the precipitation of any sediment. |
| Q05: | Reasons for media failing to solidify |
| A05: | Potential causes include excessive heating during preparation, low pH leading to acid hydrolysis of the media, inaccurate weighing, incomplete dissolution of agar, and insufficient mixing of media components. |
| Q06: | Reasons for incorrect pH values |
| A06: | Overheating during preparation, poor water quality, contamination by external chemicals, incorrect temperature during pH measurement, improper calibration of the pH meter, and low-quality dehydrated media can all contribute to incorrect pH values. |
| Q07: | Causes of precipitation in dissolved media |
| A07: | Overheating during preparation, low quality of ingredients, poor quality of dehydrated media, and incorrect pH control can lead to precipitation in dissolved media. |
| Q08: | Reasons for abnormal media color |
| A08: | Abnormal media color can be attributed to overheating during preparation, poor water quality, low-quality dehydrated media, incorrect pH, and external contamination. |
| Q09: | Causes of poor reproducibility in media inhibition |
| A09: | Overheating during preparation, poor water quality, low-quality dehydrated media, incorrect use of ingredients (e.g., inaccurate weighing, incorrect additive concentrations) can all contribute to poor reproducibility in media inhibition. |
| Q10: | What are the reasons for poor selectivity? |
| A10: | Potential causes include excessive heating during preparation, low-quality dehydrated media, incorrect formulation usage, and incorrect addition of ingredients, such as adding them to overly heated media or using incorrect concentrations. |
| Q11: | Is it better to boil and dissolve the media before autoclaving or to autoclave directly? There is confusion about this. |
| A11: | Taking MacConkey Agar as an example, autoclaving after dissolution ensures no insoluble crystal violet or purple spots on the plates. In contrast, autoclaving without prior dissolution results in crystal violet and purple spots on the plates. Therefore, the correct approach is to boil and dissolve the media before autoclaving. |
| Q12: | Why is the pH of the media not within the standard range? |
| A12: | There are several potential reasons for this, which can be broadly categorized into 6 major points: A. Poor quality (incorrect pH at the time of manufacture) B. Sterilization issues (excessive temperature or duration during autoclaving, leading to caramelization of glucose and subsequent pH drop) C. Shelf life (beyond expiration or clumping) D. Water quality (use deionized, purified, or distilled water; avoid mineral or tap water) E. Storage temperature (too high) F. Direct sunlight exposure. Any of these six factors can cause deviations in media pH. In case of such issues, it is recommended to troubleshoot each factor until the problem is resolved. |
| Q13: | Why does media char (carbonize) during autoclaving? |
| A13: | Many laboratory personnel observe charring in media after sterilization. The primary reasons for this phenomenon include: Sterilization temperature exceeding 121°C Excessive sterilization duration Overfilling the containers (should not exceed 80% of container capacity) Prolonged storage of sterilized media in high-temperature environments. |
| Q14: | How is the pH of media measured? |
| A14: | It depends on the media's sterilization requirements: For non-autoclavable media, measure the pH after boiling and cooling to 25°C (measure at least two points for solid media and take the average). For autoclavable media, measure the pH after autoclaving and cooling to 25°C (measure at least two points for solid media and take the average). |
| Q15: | Why does agar-containing media sometimes fail to solidify or solidify poorly when poured into plates? |
| A15: | For autoclavable media, ensure even shaking before pouring to distribute the agar evenly (large agar molecules tend to settle at the bottom during cooling). For non-autoclavable media, boiling and dissolving the agar once may not be sufficient; repeating the process 3-5 times ensures complete dissolution. |
| Q16: | Why is there a difference in powder color between different batches of the same product? |
| A16: | The possible reasons for this phenomenon are analyzed as follows: The medium composition contains dyes or indicators, and the water content of the powdered medium can vary between batches. Generally, the water content is required to be ≤6%, and a higher water content results in a darker color. Additionally, longer processing times also contribute to the deepening of the color. |
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