Anti-HSP70 Polyclonal Antibody
Cat: K009455P
Summary:
| 【Product name】：Anti-HSP70 Polyclonal Antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：3303/3304 | 【Swiss Prot】：P0DMV8/P0DMV9 |
| 【Tested applications】：WB,IHC,ICC,FCM,IF,ELISA | |
| 【Recommended dilution】：WB 1:500-2000. ELISA 1:5000-10000. IHC 1:100-500. FCM 1μg/Test. ICC 1:100. IF 1:100-500. | |
| 【Immunogen】：A synthetic peptide of human HSP70 | |
| 【Public Immunogen Range】：481-534/641aa | |
| 【Storage】：Store at -20℃. Avoid freeze / thaw cycles. | |
Background:
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Verified picture:
Western blot analysis with HSP70 antibody diluted at 1:300;Lane: Mouse Bladder.
Immunofluorescence analysis of A549 cells using HSP70 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cell using HSP70 antibody diluted at 1:100.